Journal: STAR Protocols

Article Title: Protocol for assessing the role of hippocampal perineuronal nets in aversive memories

doi: 10.1016/j.xpro.2021.100931

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Article Snippet: Chondroitinase ABC , Amsbio , Cat#: E1028-10.

Techniques: Recombinant, Plasmid Preparation, Blocking Assay, Cell Culture, Fluorsave, Software, Microscopy

Journal:

Article Title: Comparison of Two Fingerprinting Techniques, Terminal Restriction Fragment Length Polymorphism and Automated Ribosomal Intergenic Spacer Analysis, for Determination of Bacterial Diversity in Aquatic Environments

doi: 10.1128/AEM.01361-06

Figure Lengend Snippet: Replicated electropherograms from a single aquatic sample. (a and b) Two different T-RFLP analyses of the same sample. (c and d) Two different ARISAs of the same sample. For T-RFLP analysis, the enzyme AluI was used.

Article Snippet: About 50 ng of purified 16S rRNA gene amplicons from each of the two duplicate PCRs were digested separately in a 20-μl reaction volume that contained 10 U of the enzyme AluI (Promega) at 37°C for 3 h. This specific restriction enzyme was selected because previous studies demonstrated that it is highly efficient in assessing phylotype richness and bacterial diversity in aquatic assemblages ( 27 , 30 , 34 ).

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